In order to eliminate the effect of the sample capacitor transition current on insulating material and structure, and to improve the reliability and accuracy of the experiment results, a Leakage current detection circuit is designed for insulation life experiment based on high voltage pulse. Under the high-frequency bipolar pulse voltage, the capacitor transition current mainly focuses on the edge of pulse within 5μs; after 12μs the transition basically ends, and then the remaining part is insulation leakage current; and by use of this characteristic, the electronic switch is controlled by the controlling circuit. At each pulse edge, the capacitor transient current is removed, and instead by the leakage current value of the last cycle, while 12μs later the circuit can switch back to current the current signal. Theoretical analyzing and experimental results show that this method of leakage current detection can effectively eliminate the influence of capacitor transition current on leakage current detection circuit; at the same time, the relative deviation rate of the circuit is less than 3.03 %. It can meet the requirement of insulation life experiment based on high pulse voltage, and improve the reliability of the experiment result.