This article describes the basic functions of LabVIEW and Multisim simulation and interactive methods,and based on LabVIEW and Multisim co-simulation,we designed virtual power quality detection system which achieved by simulating a three-phase circuit voltage deviation,frequency deviation,phase imbalance and grid harmonic analysis,real-time monitoring of power quality indicators.The final results showed high accuracy based on LabVIEW and Multisim co-simulation system .It can also be used for other measurement.