Wind power generator on-line detection system can real-timely understand wind turbine operating conditions to detect hidden faults. According to the test data type wind turbine fault diagnosis is too little to misjudge the problem, this paper describes a design of multi channel data acquisition and real-time online detection device. The DSP-TMS320F28335 (F28335) system is used as the main control chip field data collection system, LabVIEW software and MCGS configuration software for synchronous display. The simulation results show that compared with the traditional wind turbine on-line detection device, can collect data under various operating conditions, and provide a reliable basis for fault diagnosis.