Based on the typical structure and electrical equipment of microgrids, this paper presents an operation and regulation manipulating scheme and develops its application. The research mainly analyzes the basic units and their characteristics of microgird operation and regulation, and establishes a standardized, reasonable and practical manipulating scheme. The scheme focuses on three kinds of microgrids’ manipulations, i.e. key equipment switching, operation mode switching and black start. The scheme builds detailed operation models for key equipment and presents the procedures of the typical manipulations. Based on the combination of the presented scheme, the field data and monitoring screen, an operation and regulation manipulating simulation platform for microgrids is developed. This simulation platform is deployed to the microgrid of an island in Zhejiang Province, which realizes the cooperation with training simulation system, operation dispatching optimization module and energy management optimization module. The application demonstrates that the scheme and the related system effectively enhance the safety and reliability of microgrid operation.