With the development of project of natural gas transmission from West to East China, in order to ensure that the pipeline is in good working condition and avoid safety accidents caused by corrosion leakage, the research of the pipeline potential collector becomes more and more important, and its calibration has a significant impact on the application of the pipeline potential collector. An automatic calibration system of the pipeline potential collector is designed and based on LabVIEW virtual instrument technology. The input and output of voltage and current, data exchange of standard signal source, digital multimeter and pipeline potential collector are controlled by computer through USB interface and RS232 serial port. The automatic calibration, data acquisition and data analysis and storage of the pipeline potential collector are realized. Through multiple fitting and contrast of calibration acquisition data by least square method, time of the calibration can be greatly shortened to improve the calibration efficiency, and the hidden safety hazards caused by misoperation in manual calibration process can be eliminated.