Aiming at the high cost and complicated production methods of the UHF RFID identification system popular in the current market, the design scheme of the UHF RFID reader compatible international standard ISO/IEC 18000-6C and EPC Class-1 Generation-2 protocol based on the RF read/write chip MagicRF M100 is proposed in this paper, and the working principle of the reader is elaborated in detail, and the more important parts of the system hardware and software design is introduced accordingly. The testing results show that the system can read, write and destroy the electronic tag. And under the gain of the 2dBi ceramic antenna, the reader recognizes the distance ideally. Meanwhile, the design of the radio frequency identification system host computer based on LabVIEW can realize the functions of data communication with the lower computer, visualization of data such as EPC and RSSI of the tag, and setting of parameters related to the reader.