Aiming at the high cost and complicated production methods of the UHF RFID identification system popular in the current market, a compatible international standard ISO/IEC 18000-6C and EPC Class-1 Generation-2 based on the RF read/write chip MagicRF M100 is proposed. The design scheme of the UHF RFID reader of the protocol elaborates the working principle of the reader, and introduces the more important parts of the system hardware and software design. After testing, the system can read, write and destroy the electronic tag. Live and other operations. And under the gain of the 2dBi ceramic antenna, the reader recognizes the distance ideally. At the same time, the design of the radio frequency identification system host computer based on LabVIEW can realize the functions of data communication with the lower computer, visualization of data such as EPC and RSSI of the tag, and setting of parameters related to the reader.